Stem Cell Therapy
Stem Cell Supernatant IV Therapy
Begin Your Journey To The Best Cell Recovery
Stem cell supernatant IV therapy is reported to support the functional recovery of damaged tissues and cells within the body, and is used for a wide range of applications — from improving non-specific complaints to age-management care.
We invite you to experience a care approach that works at the cellular level.
* AFRODE CLINIC operates on a fully referral-based model.
About
What Is Stem Cell Supernatant IV Therapy?
When stem cells are cultured, growth factors and proteins are secreted into the culture medium. The supernatant — the liquid rich in these secreted growth factors — is then purified for use. Recent research has raised expectations for the potential of these bioactive substances, including growth factors secreted by stem cells, to act on damaged cells and promote tissue regeneration.
Step 1
Active compounds such as cytokines are secreted into the culture medium during stem cell cultivation.
Step 2
After removing the stem cells, the remaining supernatant is rich in active compounds that may promote cellular regeneration.
Quality Management
Stem Cell Supernatant vs. Stem Cell Therapy: What Is the Difference?
Stem cell therapy involves harvesting a patient’s own cells, culturing them, and administering them back. However, culturing damaged cells from the patient may have limitations in terms of achievable outcomes, and the multi-step culture process carries a potential risk of bacterial contamination — cases of which have been reported.
By contrast, stem cell supernatant therapy utilizes the growth factors and exosomes secreted by stem cells to activate the patient’s own stem cells and other cells. This approach is considered to support cellular-level functional improvement with a high safety profile.
Quality Management
Our Stem Cell Supernatant: Quality Assurance
AFRODE CLINIC uses 100% Japan-sourced human adipose-derived mesenchymal stem cell conditioned medium, manufactured at a partner cell culture processing facility.
Our stem cell conditioned medium meets the quality standard of a product that holds product liability insurance (PL insurance) as a pharmaceutical — the only one of its kind in the world to have obtained this coverage.
Stem cell supernatant is an evolving field, and production methods and efficacy are understood to vary across culture facilities. Our formulation is produced using proprietary raw materials and culture technology, with a focus on balanced composition and the pursuit of clinical outcomes — and we have observed encouraging trends across a range of cases in our research process.
Safety Tests
Rigorous Quality Control
Verified safety through four independent testing protocols
01 Real-time PCR
Human Virus Testing
We conduct testing for human viruses recommended under guidelines for the quality and safety of cell-processed pharmaceutical products — including HIV1, HIV2, HCV, HBV, HTLV1&2, and Parvovirus B19. Nucleic acids are extracted using a simultaneous DNA/RNA recovery protocol with NucleoSpin RNA, and viral presence is determined by real-time PCR.
02 BacT/ALERT
Sterility Testing
We test for both aerobic bacteria (including mold) and anaerobic bacteria that may proliferate during culture. Using the BacT/ALERT sterility testing system, samples are inoculated into test culture media and incubated at 32.7°C for 7 days, with sterility determined based on microbial growth. Inhibition confirmation testing is also performed.
03 Endosafe
Endotoxin Testing
Endotoxin is a type of pyrogen (fever-inducing substance) with biological activity including pyrogenicity and potential lethality. Samples are applied to Endosafe-PTS cartridges (JP) and measured using an endotoxin detection system to assess spike recovery rates and sample endotoxin concentration.
04 MycoSEQ
Mycoplasma Testing
Mycoplasma are among the smallest self-replicating microorganisms and are known to cause conditions such as pneumonia. Using the MycoSEQ Mycoplasma Detection Kit, DNA is extracted from the sample and from a positive control spike, and mycoplasma presence is determined by real-time PCR.
Expected Effects
Potential Benefits
01
Vascular Regeneration & Angiogenesis
02
Cell Repair & Regeneration
03
Anti-Inflammatory Action
04
Free Radical Scavenging
05
Immune Modulation
06
Aesthetic Benefits
Symptoms
Conditions Where Improvement Has Been Observed
MUSCULOSKELETAL
Bone, Muscle & Joint
- Joint pain relief
- Accelerated muscle recovery
- Neuropathic pain & numbness reduction
- Inflammation suppression after injury or exercise
- Improved flexibility
- Age-related joint support
- Lower back pain & shoulder tension
MENTAL HEALTH
Mental Health Support
- Stress reduction
- Relaxation support
- Relief of anxiety & low mood
- Improved concentration
- Sleep improvement for mind-body balance
- Relief from mental fatigue
- Hormonal balance support
SYSTEMIC
Systemic Conditions
- Chronic fatigue syndrome
- Immune function support
- Headache
- Lethargy
- Dizziness & unsteadiness
- Sleep disorders
- Rheumatic conditions
DERMATOLOGICAL
Skin Concerns
- Fine lines & skin laxity
- Pore refinement & skin texture
- Dry & sensitive skin
- Vitiligo
- Dermatitis
- Pigmentation, wrinkles & dullness
- Hair thinning & alopecia support
CIRCULATORY
Circulatory Conditions
- Arrhythmia
- Peripheral circulatory disorders
- Myocardial infarction (post-care support)
- Arteriosclerosis
- Cerebral infarction (post-care support)
- Cold sensitivity relief
- Varicose vein prevention
POST-CARE
Post-Surgical & Hair Loss Support
- Post-surgical recovery support
- Wound healing support
- Alopecia improvement
- Hair follicle activation for hair growth
- Hair loss prevention
- Age-management care for neck & hands
- Liver function support & detoxification
Team
Lead Physician
Lead Physician
Dr. Shotaro Michishita
AFRODE CLINIC provides efficient, medication-minimizing recovery and preventive care — grounded in medicine — for business leaders, public figures, and others who carry significant physical and mental demands in their daily lives. We aim for this field to become a new option in healthcare, one rooted in everyday life.
Evidence
Neural Regeneration — Scientific Basis
- Stem cell supernatant has been shown to exert neural regenerative effects on pain, dysfunction, and muscle atrophy associated with nerve injury. (1)
- Stem cell supernatant has been found to prevent muscle atrophy following nerve damage, and to shift the microenvironment from a fibrotic to a self-regenerating state. (2)
(1) Li X, Guan Y, Li C, Zhang T, Meng F, Zhang J, et al. Immunomodulatory effects of mesenchymal stem cells in peripheral nerve injury. Stem Cell Res Ther 2022 Jan 15;13(1):18-021-02690-2.
(2) Wang YH, Guo YC, Wang DR, Liu JY, Pan J. Adipose Stem Cell-Based Clinical Strategy for Neural Regeneration: A Review of Current Opinion. Stem Cells Int 2019 Nov 19;2019:8502370.
Reservations & Enquiries
AFRODE CLINIC operates on a referral basis. Please contact us via the referral channel to make a reservation.
* Certain treatments (physical therapy, aesthetic procedures, Kampo herbal medicine, and nutritional support) are available without a referral.